The invention relates to a preparation method of a three-dimensional carbon nano tube/textile fiber stretchable electrode material. The preparation method comprises the following steps: coating two sides of each treated textile fiber with a graphene conducting adhesive, performing electrostatic flocking on the two sides, then putting the textile fibers in a water bath containing a penetrant, and adding a biological cationic modifier for treatment; adding sodium carbonate, adjusting the pH of a dye bath, treating and cleaning at 65-80 DEG C, and drying; putting carbon nano tubes in an acid liquor, carrying out reflux at 110 DEG C for 4-5h, to obtain acidized carbon nano tubes; at room temperature, placing the dried textile fibers in a compound prepartion liquid of a dispersing agent and the acidized carbon nano tubes, performing ultrasonic treatment, dipping and drying to obtain the three-dimensional carbon nano tube/textile fiber stretchable electrode material. The process disclosed by the invention is simple and easy for industrial production; the prepared three-dimensional carbon nano tube/textile fiber stretchable electrode material can be put into large-scale production easily; in addition, the advantages of energy saving, environment friendliness and great industrial application value are achieved.