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发明公开
CN105483108A 一种L-阿拉伯糖异构酶及其在L-核酮糖生产中的应用 有权

一种L-阿拉伯糖异构酶及其在L-核酮糖生产中的应用
摘要：
本发明公开了一种L-阿拉伯糖异构酶及其在L-核酮糖生产中的应用，本发明还公开了编码上述L-阿拉伯糖异构酶的基因序列SEQ ID NO：1，以及包含了上述L-阿拉伯糖异构酶的基因工程菌及其构建方法以及上述L-阿拉伯糖异构酶和其基因工程菌在生产L-核酮糖中的应用。该L-阿拉伯糖异构酶在温度20～70℃、pH 5.0～10.0之间对L-阿拉伯糖有着较高的催化效率，且低浓度的Mn2+和Co2+等离子的存在可大幅提升该酶的活力和热稳定性。在最适催化条件下，该酶对L-阿拉伯糖的转化率可达95％以上，该L-阿拉伯糖异构酶对于L-核酮糖的生物法生产具有十分重要的意义和经济价值。
摘要（英）：

The invention discloses L-arabinose isomerase and application thereof in production of L-ribulose. The invention further discloses a gene sequence SEQ ID NO:1 for coding the L-arabinose isomerase, a genetically engineered bacterium containing the L-arabinose isomerase and a construction method thereof and application of the L-arabinose isomerase and the genetically engineered bacterium thereof in production of the L-ribulose. The L-arabinose isomerase has higher catalytic efficiency on L-arabinose on the condition that the temperature ranges from 20 DEG C to 70 DEG C and the pH ranges from 5.0 to 10.0, and the activity and the thermal stability of the L-arabinose isomerase can be greatly promoted in the presence of low-concentration irons such as Mn<2+> and Co<2+>. Under the optimal catalytic conditions, by means of the L-arabinose isomerase, the conversion rate of the L-arabinose can reach 95 percent or above, and the L-arabinose isomerase has very important significance and economic value to biological production of the L-ribulose.

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