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发明公开
CN105483238A 转基因植物中vip3A基因的LAMP检测引物组、试剂盒及检测方法 无效 - 驳回

转基因植物中vip3A基因的LAMP检测引物组、试剂盒及检测方法
摘要：
本发明公开了一种转基因植物中vip3A基因的LAMP检测引物组、试剂盒及检测方法。引物组由4条特异性引物组成，其核苷酸序列如SEQ ID NO：1~4所示。检测试剂盒包括检测引物溶液、具有链置换活性的DNA聚合酶、10×反应缓冲液、dNTPs溶液、显色剂。检测方法是采用特异性引物及具有链置换活性的DNA聚合酶，在63~65℃对样品DNA模板进行扩增，并采用加入显色剂观察颜色变化的方法，判断样品是否含有vip3A基因成分。本发明不需要特殊仪器，具有快速高效、操作简便、特异性强等特点，适合现场检测。
摘要（英）：

The invention discloses an LAMP (Loop-mediated isothermal amplification) detection primer group and kit and a detection method for a gene vip3A of transgenic plants. The primer group consists of 4 specific primers and has nucleotide sequences shown in SEQ ID NO: 1-4. The detection kit comprises a detection primer solution, DNA polymerase with strand displacement activity, a 10x reaction buffer, a dNTPs solution and a color developing agent. The detection method comprises the steps of carrying out amplification on a DNA template of a sample at the temperature of 63-65 DEG C by adopting the specific primers and the DNA polymerase with strand displacement activity, and judging whether the sample contains a gene vip3A component or not by adopting a color developing agent added color change observing method. The detection primer group and kit and the detection method do not need special instruments, have the characteristics of rapidness, high efficiency, simplicity and convenience in operation, high specificity and the like and are applicable to field detection.

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