The invention relates to natural plant juice for brewing wine and application of the natural plant juice, and belongs to the technical field of microbial fermentation engineering. The natural plant juice is used for brewing the wine instead of sulfur dioxide. A method for preparing the natural plant juice which is aloe juice includes thoroughly cleaning fresh aloe arborescense leaves and then removing the skins of the fresh aloe arborescense leaves; cutting the fresh aloe arborescense leaves to obtain small dices; smashing the small dices by the aid of a juice extractor to obtain juice; filtering the juice by the aid of four layers of medical gauze to obtain filter liquid. The aloe juice can be applied to brewing the wine after grapes are selected, stems of the grapes are removed and the grapes are crushed, the mass ratio of the aloe juice added into grape juice is 3-8%, and the aloe juice and the grape juice are uniformly mixed with each other and are fermented. The natural plant juice and the application have the advantages that the aloe juice is used as an antibacterial agent for fermenting the wine instead of sulfur dioxide which is used as an antibacterial agent in the traditional processes for producing wine, and accordingly the safety and the quality of the wine can be improved.