The invention belongs to the field of fermentation engineering and particularly relates to a method for producing norvancomycin by fermentation. In this method, by adding super-paramagnetic microspheres in a norvancomycin synthesis phase to adsorb norvancomycin in a fermentation broth, a reduction is given to the feedback inhibition of a fermentation product upon generated bacteria and an improvement is given to the biological synthesis of norvancomycin. After fermentation, fermentation unit of the norvancomycin is up to 22593 ug/ml; compared with a method adding no super-paramagnetic microspheres, this method provides an increase of greater than 35% in norvancomycin yield, an improvement in productivity and equipment utilization and a reduction in wastewater and waste residue discharge.