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发明公开
CN105543376A 赭曲霉毒素A的快速检测方法 无效 - 驳回

赭曲霉毒素A的快速检测方法
摘要：
本发明涉及一种赭曲霉毒素A的快速检测方法，该方法包括如下步骤：（A）使赭曲霉毒素A核酸适体（Apt）与单链信号探针DNA（ssDNA）杂交，形成杂交链；（B）使该杂交链与待测样品接触，当待测样品中有赭曲霉毒素A存在时，杂交链与赭曲霉毒素A反应释放出单链信号探针ssDNA；（C）利用DNA扩增，使杂交链成双链DNA，然后用外切酶选择性地催化双链DNA水解成单核苷酸，而单链信号探针ssDNA不水解而保留下来；（D）在ssDNA诱导下，铜离子还原生成近红外荧光铜纳米粒子；检测体系荧光强度，从而测定待测样品中的赭曲霉毒素A的含量。该方法具有高灵敏度，操作简单，低成本等特点。
摘要（英）：

The invention relates to a rapid detection method for ochratoxin A. The method comprises the following steps that A, an aptamer (Apt) of the ochratoxin A is hybridized with single-stranded signal probe DNA (ssDNA) to form a hybridized chain; B, the hybridized chain is made to make contact with a sample to be tested, and when the ochratoxin A exists in the sample to be tested, the hybridized chain and the ochratoxin A are subjected to a reaction to release the single-stranded signal probe DNA (ssDNA); C, DNA amplification is utilized for making the hybridized chain into a double-stranded DNA, then, an excision enzyme is used for selectively catalyzing the hydrolysis of the double-stranded DNA to form mononucleotide, and the single-stranded signal probe DNA (ssDNA) is not hydrolyzed and reserved; D, under the induction of the ssDNA, copper ions are reduced to generate near infrared fluorescence copper nanometer particles; the system fluorescence strength is detected, and therefore the content of the ochratoxin A in the sample to be tested is tested. The method has the advantages of being high in sensitivity, easy to operate, low in cost and the like.

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