The invention discloses a polypeptide sequence capable of specifically being bound to fumonisin B1. The polypeptide sequence is five phage displaying peptides, which are composed of 12 amino acids. The provided phage displaying peptides, which can be bound to FB1, have high affinity and specificity to FB1; and compared with the conventional monoclonal antibody, the method of choosing phage displaying peptide that can be bound to target from a phage displaying random polypeptide library has the advantages of simple operation, and easiness for massive preparation and purification.