The invention discloses an extraction method of active ingredients of isodon amethystoides and belongs to the technical field of extraction of active ingredients of plants. Firstly, isodon amethystoides roots are treated with the press-shear assisted interaction technology, coarse powder of the isodon amethystoides roots and a chemical aid are subjected to superfine grinding and sieved, then ethanol is added, and the mixture is evenly mixed; after ultrasonic extraction, an extraction solution is subjected to reduced pressure distillation, the ethanol fraction is recycled, a concentrated solution is extracted with petroleum ether, chloroform, ethyl acetate and n-butyl alcohol respectively, an extract solution is subjected to reduced pressure concentration, fractions are recycled, and condensed dry powder is a crude extract with different phases. Detection and analysis on different pharmaceutical activity of the crude extract are performed to determine target ingredients. As the new technology for extracting the active ingredients of the isodon amethystoides, the extraction method can effectively extract the active ingredients of the isodon amethystoides and can more effectively extract the active ingredients compared with a traditional extraction method. Ultrasonic wave assisted extraction is used, the dissolution time of the active ingredients is greatly shortened, and the extraction efficiency is improved. The method is simple in process, can save the cost and is more beneficial to industrialization.