The invention provides a heat exchange device. The heat exchange device is provided with an exchange conveying path, a plurality of bearing parts and a plurality of constant-temperature regions, wherein the plurality of bearing parts are used for bearing a plurality of bearing plates in one to one correspondence, and each bearing plate is provided with a plurality of application containers; the plurality of constant-temperature regions are arranged along the exchange conveying path and are used for partially covering and containing the plurality of bearing parts; the temperature of at least one in the plurality of constant-temperature regions is different from that of the other constant-temperature region; the plurality of bearing parts are equal to the plurality of constant-temperature regions; the plurality of constant-temperature regions are subjected to heat exchange with the plurality of bearing plates at the same time; the plurality of bearing parts carry the plurality of bearing plates to move synchronously along the sealed exchange conveying path to pass through the constant-temperature regions; and the plurality of bearing plates pass through the plurality of constant-temperature regions one by one. Moreover, a gap is formed between holding assemblies of each two adjacent bearing plates provided by the invention. The gaps ensure that matter to be detected is surrounded by an exchange medium so that the temprature of all regions of the matter to be detected is uniform.