The invention discloses a detection method for transgenic ingredients in tomato sauce, and belongs to the technical field of biology. The method includes the steps that the transgenic ingredients, endogenous standard genes and exogenous standard genes are determined; a multiple amplification primer is prepared; sampling and mixing are conducted to obtain a mixed sample; a genome in the mixed sample is extracted, and the exogenous standard genes are added to obtain mixed nucleic acid; a high-throughput sequencing library is constructed; high-throughput sequencing is conducted on the high-throughput sequencing library to obtain a sequencing fragment set; the number of sequencing fragments of the transgenic ingredients in the sample to be detected, the number of sequencing fragments of the endogenous standard genes and the number of the sequencing fragments of the exogenous standard genes are obtained; according to the number of the sequencing fragments of the exogenous standard genes, whether an experiment is successful or not is judged, and the content of the transgenic ingredients in the sample to be detected is calculated; according to the content of the transgenic ingredients, whether the sample to be detected contains the transgenic ingredients or not is judged. Multiple transgenic ingredients in the sample to be detected can be quantitatively detected at the same time.