The invention discloses research on application of isoorientin of babysbreath to preparing medicines for treating immunological hepatic fibrosis. The isoorientin of the babysbreath is an active ingredient of traditional Chinese medicines. The research on the isoorientin of the babysbreath in the aspect of treating the immunological hepatic fibrosis is carried out for the first time, as shown by animal experiments, the isoorientin of the babysbreath can act on a plurality of links or a plurality of targets of immunological hepatic injury, and effects mainly can be realized by the aid of NF-kB signal transduction pathways. The research has the advantages that lipid peroxidation, collagen deposition and matrix metalloproteinase can be effectively suppressed, pathological injury of hepatic tissues can be effectively alleviated, the NF-kB signal transduction pathways can be effectively blocked, accordingly, the hepatic injury can be obviously alleviated, and the isoorientin of the babysbreath has unique merits for preventing and treating the hepatic injury; obvious suppressing effects can be realized by the isoorientin of the babysbreath for hepatic injury due to porcine serum, and the novel medicines with potential value can be used for treating the immunological hepatic fibrosis.