The invention discloses a manufacturing method of a seven-electrode conductivity sensor based on the MEMS silicon-glass technology. The manufacturing method comprises the steps that metal electrodes are deposited on an N-type silicon chip, the metal electrodes are deposited on a glass sheet, and gold-gold bonding is performed on the end surfaces of the metal electrodes so that a cylindrical cavity is enabled to form a sealed conductive pool. The manufacturing method of the seven-electrode conductivity sensor is simple, the size is small, mass production can be realized based on the MEMS processing technology, production cost is reduced, processing precision is high, reliability is high and universality is high.