The invention belongs to the field of medical preparations and relates to an antibacterial hemostatic adhesive plaster taking pimpinellin serving as a natural active component as a main drug and a preparation method of the antibacterial hemostatic adhesive plaster. The plaster consists of a backing layer made of a backing material, a drug-loaded pressure-sensitive layer containing the pimpinellin and an adhesion resisting layer; acrylate pressure-sensitive adhesives are used as a plaster matrix; peppermint oil, azone and isopropyl myristate are jointly used to serve as a skin penetration enhancer, wherein optimal doses of the peppermint oil, the azone and the isopropyl myristate are 12 percent, 6 percent and 4 percent of the dosage of a formula separately; by using the skin penetration enhancer, the absorption of the pimpinellin serving as the active component is effectively promoted; the prepared plaster is uniform in film formation and is moderate in viscosity; the drug is quickly and completely released; meanwhile, the influence of solvent addition on the adhesiveness of the pressure-sensitive adhesive is avoided. The research on pharmacodynamics shows that the hemostatic adhesive plaster has a good hemostatic effect which is equivalent to that of Yunnan Baiyao. The antibacterial hemostatic adhesive plaster and the preparation method thereof disclosed by the invention have the advantages of simple and convenient process, convenient operation, capability of realizing industrial production and good market prospect.