The invention discloses an application method of a microorganism multi-parameter comprehensive test platform. A high-precision plunger type micro-scale sample feeding pump is used to precisely control the sample feeding of a microorganism test solution so as to stabilize the feeding quantity of the microorganism test solution during the whole test process and allow the microorganism test solution to enter from a liquid inlet, pass a micro-flow channel and discharge from a liquid outlet. A lens hole is connected with a microscopy hyper-spectrometer, and a background light source is turned on to provide background light for the microscopy hyper-spectrometer through a background light hole so as to collect microscopy hyper-spectrometer image information of microorganisms. Capacitive dielectric electrodes, planar waveguide and comb type electrodes and parallel electrodes are respectively connected with dielectric spectrum test equipment to detect the dielectric characteristics and number of microorganisms. An optical fiber through hole is connected with an optical fiber spectrometer to detect the spectral characteristics of the microorganisms. The microorganism multi-parameter comprehensive test platform is applicable to qualitative and quantitative detection of microorganisms in liquid, multi-information of the microorganisms can be synchronously collected, and detection efficiency and precision are increased.