The invention discloses a method for measuring the content of allicin by use of a sodium saccharin standard substance instead of an allicin standard substance. The method comprises the steps of: drawing standard curves of allicin and sodium saccharin respectively, calculating a correction factor between the allicin and the sodium saccharin to obtain the corresponding relation of the allicin and the sodium saccharin, and measuring the content of allicin of a sample to be detected by use of the sodium saccharin standard substance according to the corresponding relation. According to the method, the sodium saccharin standard substance instead of the allicin standard substance is used for measuring the content of the allicin, the testing error caused by instability of the allicin standard substance is avoided, the sodium saccharin standard substance is cheaper than the allicin standard substance and can be purchased easily, and the cost is saved.