The invention discloses a method for evaluating inhibition of marine fish mucus lectin for paralembus digitiformis, and belongs to the technical field of aquatic product disease control. The method comprises activity inhibition evaluation and combining evaluation of Lily-type lectin protein for paralembus digitiformis. By means of the method, the inhibition of the marine fish Lily-type lectin (LTL) protein for the activity of paralembus digitiformis can be easily and conveniently evaluated, and the combining phenomenon of the LTL protein and paralembus digitiformis can be clearly observed through a fluorescence microscope.