The invention discloses a front-back movable, up-down adjustable and left-right center precise positioning and clamping device, belongs to the field of positioning and clamping devices, and solves the problems of complex structure and difficult adjustment in a traditional positioning and clamping device. The device comprises a guide rail, a bracket platform, an up-down adjusting cushion plate and a left-right center precise adjusting device, wherein the guide rail is fixedly arranged on mechanical equipment or a working platform; the bracket platform is movably mounted on the guide rail, and can perform a reciprocating motion along the guide rail; the up-down adjusting cushion plate is fixedly arranged at the middle part of the top surface of the bracket platform, and is adjustable in thickness; and the leftright center precise adjusting device is fixedly mounted on the bracket platform, and is used for adjusting a left-right position of a workpiece on the working platform and clamping and fixing the workpiece.