The invention discloses a method for quality detection of Wuli cold dispelling powder. The method includes thin layer chromatography identification and content determination of hesperidin in the Wuli cold dispelling powder. Thin layer chromatography identification includes thin layer chromatography identification of Fructus Zanthoxyli and Semen Cleomes Gynandrae. Chromatographic conditions of content determination of the hesperidin include: octadecylsilane chemically bonded silica serves as a filler; methanol is taken as a mobile phase A and [glacial acetic acid: water (1:300)] is taken as a mobile phase B for gradient elution; a detection wavelength is 284nm; a flow rate is 1.0mL.min<-1>; a detector is an ultraviolet detector; a column temperature is 30 DEG C; a sample size is 5 microliters. A reference solution and a test solution are injected into a liquid chromatographic column, and test is carried out according to the conditions to record 70min chromatogram. The method is simple, quick, accurate and high in reproducibility and provides quality basis for comprehensive assessment and control of the Wuli cold dispelling powder.