An operation method of a calibration guidance system includes a feature extraction unit executing a feature extraction operation on a first image group including a first object captured by a multi-camera system to generate a first feature point group corresponding to a predetermined position within an image capture range of the multi-camera system; and a guidance unit determining whether to generate a direction indication to guide the first object to another predetermined position within an image capture range according to a first comparison result between a block corresponding to feature points of the first feature point group of the predetermined position and a predetermined block when a number of the feature points of the first feature point group is greater than a predetermined number.