The invention provides a kit for early prediction of osteoporosis. The kit comprises a detection plate, a standard substance, interferon-gamma antibodies I and interferon-gamma antibodies II capable of emitting fluorescent light, wherein the detection plate comprises a plate body and at least two sample adding holes in the plate body; the bottoms of the sample adding holes are coated with the interferon-gamma antibodies II; the standard substance is an interferon-gamma water solution, and the concentration of the water solution is higher than or equal to 1 ng/mL. A serum sample of a to-be-tested person and the standard substance are added to the sample adding holes in the detection plate respectively, and whether the to-be-tested person has the risk of osteoporosis is judged according to the difference of fluorescence intensity. The kit is low in cost, high in accuracy and good in reliability, has a risk prediction function by comparison with traditional bone density examination and can provide a diagnosis basis for prediction of osteoporosis in clinic.