The invention discloses a method for increasing the alkaline pretreated wood fiber raw material enzymolysis yield. The method comprises the steps of raw material pretreatment and enzyme hydrolysis, wherein in the step of pretreatment, polyethylene glycol diglycidyl ether is added to co-treat a substrate. The method for increasing the alkaline pretreated wood fiber raw material enzymolysis yield has the beneficial effects that during low-temperature alkaline pretreatment, polyethylene glycol diglycidyl ether is added to co-treat the substrate, thus effectively weakening nonspecific adsorption of lignin toward cellulase in the enzyme hydrolysis process and being beneficial to promoting cellulase hydrolysis; test results show that compared with existing research results, the enzyme hydrolysis yield is increased by 39.75% and reaches 71.26%; meanwhile, the method is simple in pretreatment process conditions, mild in reaction conditions and high in carbohydrate recovery rate, has the advantages of low energy consumption, medicine recovery, and the like, further has actual operation value and has good practicability.