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发明公开
CN105779649A 一种检测禽白血病病毒的免疫PCR试剂盒 无效 - 驳回 复审申请

一种检测禽白血病病毒的免疫PCR试剂盒
摘要：
本发明属于生物技术检测领域，具体涉及一种检测禽白血病病毒的免疫PCR试剂盒。所述试剂盒包括：两个抗体寡核苷酸探针、连接反应液、DNA连接酶、蛋白酶、Fast Master混合液和Universal qPCR反应液。本发明利用生物素标记的抗禽白血病病毒特异性抗体以及商品化邻位连接试剂，通过免疫PCR检测样品中禽白血病病毒抗原。实现不用对样品进行繁琐的核酸提取过程，通过PCR方法对禽白血病病毒抗原进行快速高通量检测。这一检测禽白血病病毒免疫PCR试剂盒将应用于禽白血病病毒临床检测与净化中，将填补国内外相关技术空白。
摘要（英）：

The invention belongs to the field of biological technical detection and particularly relates to an immune PCR reagent kit for detecting avian leukemia virus. The reagent kit comprises two antibody oligonucleotide probes, a coupled reaction solution, DNA ligase, protease, a Fast Master mixed solution and a Universal q PRC reaction solution. The immune PCR reagent kit uses a biotin-labeled avian leukemia virus preventing special antibody and a commercialized ortho-position connecting reagent, can detect avian leukemia virus antigen through immune PCR and can quickly detect the avian leukemia virus antigen in a high-throughout mode through the immune PCR without carrying out a complex nucleic acid extracting process on a sample. The immune PCR reagent kit for detecting the avian leukemia virus can be applied to clinical detection and purification of avian leukemia virus and will fill domestic and oversea correlated technique blank.

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