The invention discloses test paper for detecting residual triadimefon in crops and application and a preparation method of the test paper.The test paper comprises a sample absorbing pad, a conjugate releasing pad, a reaction film, a water absorbing pad and a base plate; the reaction film is provided with a detection line (T line) coated with a triadimefon coupling antigen and a quality control line (C line) coated with a goat-anti-mouse antibody, and the conjugate releasing pad is coated with a triadimefon monaclonal-colloidal gold marker.The test paper has the advantages of being high in sensitivity and specificity, low in cost, easy to operate, short in detection time, wide in application range, easy to store and long in quality guarantee period, is an ideal and rapid screening means, can better meet the requirements for on-site rapid detection and has the good application and popularization prospects.