The invention discloses a method for separating chromone compounds from Chinese eaglewood.The method comprises the steps that ethanol is added to Chinese eaglewood powder, heating reflux is conducted, and an extract is obtained after concentration is conducted; the extract is suspended in water and extracted with dichloromethane and ethyl acetate, and a dichloromethane extract and an ethyl acetate extract are obtained; the dichloromethane extract and the ethyl acetate extract are subjected to silica-gel column chromatography and semi-preparative high performance liquid chromatography separately, and the chromone compounds are obtained, wherein the chromone compounds as shown in formulas 1-5 are 5-hydroxy-7-methoxy-2-[2-(4'-methoxyphenyl)ethyl]chromone, 5alpha,6alpha-epoxy-7beta,8alpha,5'-trihydroxy-4'-methoxy-2-(2-phenethyl)chromone, 7,8-dihydroxy-2-[2-(5'-hydroxy-4'-methoxy-phenyl)ethyl]chromone, 5,8-dihydroxy-6-methoxy-2-(2-phenethyl)chromone and 6,7-dimethoxy-2-[2-(4'-methoxyphenyl)ethyl]chromone respectively.The method is simple and easy to operate, and four novel chromone compounds and a chromone compound with the known structure are successfully prepared, wherein the first compound and the fifth compound have significant anti-inflammatory properties.