The invention discloses a preparation process for a low-temperature phase-change nano cold storage material having a bacteriostatic effect, wherein the preparation process includes the steps of preparation of nano silver particles, preparation of an organic/inorganic cold storage liquid, preparation of a low-temperature phase-change material, low-temperature treatment and packaging and storage and the like. An eutectic salt solution composed of various inorganic salts and an organic macromolecular phase-change material are adopted for preparing the low-temperature phase-change material having high crystallization enthalpy value and excellent heat conduction effect and having the characteristics of good stability, difficult decomposition, relatively low cost, safety, no toxicity, a certain plasticity and the like; moreover, the cold storage material can effectively extend the shelf-life of aquatic products by 2-3 days. At the same time, nano metal particles having antibacterial and bacteriostatic effects are added, so putrefying bacteria carried by the aquatic products can be effectively killed, even growth and propagation of potential food-borne pathogenic bacteria can be effectively inhibited, and thus the quality and the quality safety of the aquatic products can be facilitated to be improved.