A preparation method of anti-dazzle glass includes the steps of: (a) depositing a layer of ZnO thin film on a surface of a glass substrate; (b) etching the ZnO thin film with an acid solution or an alkaline solution in low concentration to form a three-dimension mask plate; and (c) etching the glass with the three-dimension mask plate ZnO thin film formed thereon with plasma to prepare the glass having an uneven structure. In the method, the anti-dazzle glass is prepared by pre-preparing the three-dimension mask plate and then performing plasma etching. The method has high etching speed and uniform etching pattern and can be used for forming a uniform uneven structure. The anti-dazzle glass has excellent anti-dazzle performance, has low damage on human body and is suitable for a high-grade display panel.