The invention provides a method for detecting Psa in kiwi fruit pollens. The method comprises the following steps: extracting DNA from pollen mixed bacterial suspension; designing two pairs of specific primers according to hrpW gene of kiwi fruit Psa, carrying out nested-PCR, amplifying a pollen sample to obtain an outer primer amplified band with a size of about 590 bp and an inner primer amplified band with a size of about 240 bp; and finally carrying out sequencing to verify the Psa. The detection sensitivity is 103 cfu/mL. The provided Psa rapid detection method has the advantages of stronger specificity, higher stability, and higher sensitivity, can differentiate pathogenic bacteria having close ties of consanguinity with Psa such as pseudomonas syringae pv.Theae, pseudomonas avellanae, and the like, and is capable of reducing the false positive results.