The invention discloses an adenovirus vector for specifically inhibiting proliferation and migration of smooth muscle cells and application of the adenovirus vector. Target genes and miRNA regulatory sequences are inserted at multiple clone sites of the adenovirus vector. After atherosclerosis disease interventional therapy, a main principle of restenosis lies in that the smooth muscle cells migrate to an intima and perform pathological proliferation. The adenovirus vector disclosed by the invention is applied, so that the movement of the proliferation, the migration and the like of the smooth muscle cells can be well restrained, and restenosis can be avoided. The adenovirus vector is adjusted and controlled based on miRNA, so that the purpose of specifically inhibiting proliferation and the migration of vascular smooth muscle cells can be realized, and influence on vascular endothelial cells is little. Therefore, the repair of endothelium is not influenced, so that the risk of thrombosis is reduced, and the use of antiplatelets and bleeding can be reduced; the effect of interventional treatment of the atherosclerosis diseases can be improved.