The invention relates to the field of spinning yarns, in particular to a preparation and processing method of anti-bacterial knitting yarns. The invention aims to provide anti-bacterial knitting yarns which can achieve 3A level on the anti-bacterial effect and further reduce production cost and a preparation and processing method of the anti-bacterial knitting yarns. The preparation and processing method comprises the following step: step one, selection of raw materials: selecting the following raw materials in percentages by weight: 22-29% of wool, 30-45% of mucilage glue, 25-35% of chinlon and 5-7% of Kapron copper ion fibers, wherein the sum of the percentages of the wool, the mucilage glue, the chinlon and the Kapron copper ion fibers is 100%. According to the preparation and processing method of the anti-bacterial knitting yarns designed by the invention, the wool, the mucilage glue, the chinlon and the Kapron copper ion fibers are adopted, the Kapron copper ion fibers are dyed and reduced at first so that the fastness of the Kapron copper ion fibers meets requirements, and then the yarns which reach the national 3A-level anti-bacterial standard can be processed according to the specified processing steps.