The invention discloses a brucellosis vaccine needle-free injection system and application. The brucellosis vaccine needle-free injection system comprises a container filled with a brucellosis vaccine and a readable carrier. Content recorded in the readable carrier includes that needle-free injection dosage of the brucellosis vaccine is not larger than the standard dosage of the brucellosis vaccine. Experiments prove that when a needle-free injection ampoule and a needle-free syringe are combined and used for immunization, the antigen dosage only accounts for part of a traditional intramuscular injection antigen dosage, and the same or better immune effect can be achieved. It is further indicated that a needle-free immune mode is an ideal strategy capable of being selected by the brucellosis immunization vaccine.