The invention provides a method for detecting cattle SMC2 gene recessive lethal mutation, and relates to the field of molecular biology. The method provided by the invention is based on a pyrosequencing technology, a pair of primers, a universal primer and a sequencing primer having the amino acid sequences as shown in SEQ ID No.1, 2, 3 and 4 respectively are adopted for analyzing single nucleotide polymorphism of a 95410507 bp locus of a cattle No.8 chromosome, and results find out that a wild-type homozygote has a T/T genotype at a mutation locus, a pyrosequencing result pattern indicates that the peak height ratio of the T base to the C base is 3:1; a lethal mutation carrier peak pattern has a T/C genotype at the mutation locus, and the pyrosequencing result pattern indicates that the peak height ratio of the T base to the C base is 5:3. The method provided by the invention is simple to operate, high in sensitivity, strong in accuracy, high in throughput and low in detection cost, and has importable application value in cattle breed-conservation and breeding processes.