The invention provides a kit for testing PG (pepsinogen) I or II. The kit comprises magnetic particles for testing of PG I/II, tracing conjugates for testing of PG I/II and a PG I/II calibrator, wherein the magnetic particles for testing of PG I/II are magnetic microspheres labeled with PG I/II monoclonal antibodies and a magnetic particle preserving fluid; the tracing conjugates for testing of PG I/II are tracing markers labeled with PG I/II antibodies and tracing conjugate diluent, and the tracing markers are acridinium ester and derivatives of acridinium ester; the PG I/II calibrator comprises a PG I/II solution and is used for calibrating a principal storage curve in a tester. The kit is combined with a high-sensitivity chemiluminiscence testing technique and a magnetic particle separation technique on the basis of enzyme immnunoassay, paramagnetic particles are taken as solid-phase carriers, the reaction area is increased due to the small particle size and the large specific area, and the sensitivity is greatly improved; a full-automatic instrument and matched reagents are used, artificial factors are reduced to the largest extent, the method stability and the result repeatability are improved, and the difference in a batch and the difference between batches are smaller.