The invention relates to a three-degree-of-freedom operation mechanism used for tactile feedback. The operation mechanism comprises a handle (2), a disc damper (3) and two linear dampers, wherein the disc damper (3) and the linear dampers are all magneto-rheological dampers; the disc damper (3) is coaxially connected with the handle (2); one end of each linear damper is rotationally connected with the handle (2), and the other end of the linear damper is rotationally connected with a fixed object; the damping values of the magneto-rheological dampers are controlled by a computer in a tactile feedback system; the disc damper (3) applies rotational resistance to a spinning motion of the handle (2); and the linear dampers apply linear resistance to a translational motion of the handle (2). Compared with the prior art, the operation mechanism has the advantages of wide motion range, flexibility in motion, easiness in control, flexible structure, light weight, easiness in installation and replacement, and the like.