The invention discloses a triazole norcantharidin derivative containing Arabinoside structure and a preparation method and application thereof. The preparation method is as below: using 1,3-dipolar cycloaddition method to introduce 1,2,3-triazole into sites C5 and C6 in norcantharidin structure, and reacting with triacetyl arabinose azide to introduce Arabinoside structure, so as to order to synthesize the triazole norcantharidin derivative containing Arabinoside structure. The compound has a variety of biological activities and can be used for preparing antitumor drugs.