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发明授权
CN106085995B 一种基因定点改造的蛋白质二硫键异构酶及其应用 有权 转让

一种基因定点改造的蛋白质二硫键异构酶及其应用
摘要：
本发明公开一种基因定点改造的小麦蛋白质二硫键异构酶及其应用，属于基因工程领域。本发明通过对野生型小麦蛋白质二硫键异构酶进行基因定点改造，获得了仅含有伴侣活性的小麦蛋白质二硫键异构酶突变体，其氨基酸序列如SEQ ID NO：4所示，是由氨基酸序列为SEQ ID NO：3所示的野生型小麦蛋白质二硫键异构酶定点改造而得，其43、46、387和390位氨基酸由半胱氨酸(Cys)突变成丝氨酸(Ser)。面包烘焙结果表明，基因定点改造的小麦蛋白质二硫键异构酶在改善面包品质效果上优于野生型小麦蛋白质二硫键异构酶。研究结果为深入揭示wPDI的伴侣活性对面筋蛋白的作用机制奠定了基础。
摘要（英）：

The invention discloses a gene fixed point reconstructed wheat protein disulfide bond isomerase and a use thereof and belongs to the field of gene engineering. Through gene fixed point reconstruction of a wild-type wheat protein disulfide bond isomerase, a wheat protein disulfide bond isomerase mutant only with chaperone activity is obtained and has an amino acid sequence shown in the formula of SEQ ID NO: 4. The amino acid sequence is obtained through fixed point reconstruction of a wild-type wheat protein disulfide bond isomerase with an amino acid sequence shown in the formula of SEQ ID NO: 3 and amino acids at 43th, 46th, 387th and 390th sites of the amino acid sequence shown in the formula of SEQ ID NO: 4 are mutated into serine (Ser) from cysteine (Cys). A bread baking result shows that the wheat protein disulfide bond isomerase subjected to gene fixed point reconstruction has bread quality improvement effects better than those of the wild-type wheat protein disulfide bond isomerase. The research result lays a foundation of deep reveal of a mechanism of wPDI chaperone activity action on gluten.

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