The invention specifically relates to a determination method for the effective content of gelatine in a borofluoride solution for electrodeposition of lead, belonging to the technical field of battery production. The method comprises the following steps: removing lead ions in the solution for electrodeposition of lead, then carrying out neutralization so as to allow the solution to be neutral and determining the content of nitrate ions by using ultraviolet-visible spectrophotometry; and sucking up a sample of the solution for electrodeposition of lead, placing the sample into a beaker, adding a tannic acid solution under continuous stirring, carrying out standing and filtering, washing obtained precipitate until the precipitate is neutral, drying the precipitate in a baking oven to a constant weight, weighing the weight of the precipitate and calculating the content of gelatine in the sample. The method provided by the invention can accurately determine the contents of nitrate impurity and gelatine in the borofluoride solution for electrodeposition of lead, overcomes the problem of great errors in detection of the content of gelatine in the solution, is applicable to guidance for maintenance of a process for electrodeposition of lead with borofluoride, improves stability of the electrodeposition solution, saves production cost and reduces environmental pollution.