The invention discloses a preparation method of a chilled chicken breast, and belongs to the field of chicken breast preservation. The preparation method of the chilled chicken breast comprises the following steps: taking a newly killed chicken breast and soaking, carrying out freezing-thawing circularly, and performing ultrahigh pressure sterilization and modified atmosphere packaging. Before the preparation process of the circular freezing-thawing, the surface of the chilled chicken breast is uniformly coated with chayote pectin. The preparation method of the chilled chicken breast disclosed by the invention has the characteristics of capabilities of well preserving taste substances and flavor substances in the chicken breast and reducing the loss of inosinic acid and total free amino acid in the chilling process, good sensory meet quality and further prolonged shelf life of the chilled chicken breast to ensure the meat quality.