The invention relates to the technical field of cell culture, in particular to a composition and application thereof to CIK cell culture. Through the cooperation of the composition and a culture method, the yield of CIK cells can be improved, and the ratio of CD3+CD56+ double positive cells is high. It is shown through experiments that the composition and the culture method induce single karyocyte, the total number of cells can be increased to 8.9*10<9> from the before-induction total number of 5*10<7>, the ratio of CD3+CD56+ double positive cells is 36.1%, and the effect is remarkably better than that achieved when IL-2 (P is smaller than 0.05) is independently used.