The invention discloses an anti-drop teaching glass slide used for single cell gel electrophoresis. The glass slide comprises a slide glass body and two extension plates respectively connected with two ends of the slide glass body. Each extension plate is provided with a hole. The bottom of each extension plate is provided with a notch. A placing groove is arranged in the middle of the slide glass body. Two grooves are arranged on the outer wall of one side, which is close to the placing groove, of the slide glass body. The inner wall of each groove is rotationally connected with a glass cover plate through a rotation shaft connector. A glass cover piece and a sealing ring are arranged on each glass cover plate, wherein the glass cover piece and the sealing ring match the placing groove. Each sealing ring sleeves the outer wall of a glass cover piece. According to the invention, the glass slide can be conveniently taken; each extension plate is provided with a hole groove which fits a finger; each extension plate is provided with a non-slip rubber thread to prevent the slide glass from slipping off; gel can be placed in the placing groove; the glass cover pieces and the sealing rings match the placing grooves to lock the gel and effectively prevent the gel from overflowing; an objective is protected; and the anti-drop teaching glass slide has the advantages of simple operation and high practicality.