The invention relates to an experimental system for controlling a cell to rotate in a three-dimensional mode and a working method thereof. The experimental system comprises a micro-fluidic chip, an alternating signal source and a light source for generating corresponding light graphs, and the alternating signal source is connected into the micro-fluidic chip and is projected through the corresponding light graphs to adjust the cell posture and/or position in the micro-fluidic chip. The experiment system is suitable for the method of rotating the cell in the three-dimensional mode and then adjusting the posture, and it is avoided that a complex physical entity electrode array or other contact manipulating components are manufactured on the chip.