The invention provides a canine distemper virus colloidal gold immunochromatographic test strip and a preparation method thereof, relates to the technical field of colloidal gold test strip, and solves the problems of complex test procedures, time consumption, professional operation requirement and dependence on the laboratory in the existing CDV detection techniques. The test strip includes a PVC base plate, and a sample pad, a gold-labeled combined pad, a nitrocellulose membrane with a detection line and control line, and an absorption pad, which are pasted on the base plate. The gold combined pad is wrapped with a coupling marker of purified canine distemper virus monoclonal antibody N18 and colloidal gold; the detection line on the nitrate cellulose membrane is wrapped with a purified canine distemper virus purified monoclonal antibody C42; and the control line on the nitrocellulose membrane is coated with a goat-anti-mouse IgG. The test strip of the invention has the advantages of simple and rapid operation, clear and easy detection, strong specificity, high sensitivity, and no need of instruments and equipment.