The invention discloses an experimental system for monitoring release of pollutants from the bottom sediment in a flood process, the experimental system includes a pollution sand sample mixing device, a sand wave preparation monitoring device and a sampling device, the pollution sand sample mixing device includes a sand mixing container used for mixing water sand and the pollutants, a water sand mixing box used for storing a water sand mixture and a sewage pool used for storing the pollutants, the water sand mixing box is connected with the top of the sand mixing container by a conduit, the sewage pool is respectively connected with the top and the bottom of the sand mixing container by water conduits, the sand wave preparation monitoring device comprises at least one detachable sand wave mold and an electric conductivity probe placed in the sand wave mold, and the sampling device comprises a sampler and a plurality of removable sampling plates, wherein the sampler is provided with an upper opening and a lower opening, and is assembled by the plurality of removable sampling plates. The experimental system adopts the manner of vacuum pump and conduit drainage combination, and uses the simple inverted siphon physical principle, the water sand in the water sand mixing box is sucked into the sand mixing container, and a great deal of manpower and time are saved.