A method for preparing jet fuel with animal and vegetable fat includes the steps that in a hydrotreating reaction zone, vegetable oil and/or animal fat are/is mixed with hydrogen and regulation gas, then the mixture makes contact with a hydrotreating catalyst for a reaction, and a hydrotreating reaction product is obtained; in a hydroisomerizing pour point reduction reaction zone, the obtained hydrotreating reaction product makes contact with a hydroisomerizing pour point reduction catalyst for a reaction, and a hydroisomerizing pour point reduction reaction product is obtained, wherein a hydroisomerizing pour point reduction zone A and a hydroisomerizing pour point reduction zone B which are connected in series are arranged in the hydroisomerizing pour point reduction reaction zone; in a hydrofining reaction zone, the hydroisomerizing pour point reduction reaction product makes contact with a hydrofining catalyst for a reaction, and a hydrofining reaction product is obtained; the hydrofining reaction product is subjected to separation and fractionation, and then the jet fuel is obtained. By means of the method, the obtained jet fuel is high in yield and low in freezing point.