The invention belongs to the technical field of medicines and provide a percutaneous absorption preparation containing a glycyrrhetinic acid 30-amide derivative and a preparation method and application thereof. The percutaneous absorption preparation is ointment, gel, emulsion, a film agent, liniment, transdermal patch and other pharmaceutical acceptable percutaneous absorption dosage forms prepared by adding appropriate pharmaceutic adjuvants to an active ingredient TY-501, wherein the weight percentage of medicinal active ingredient per unit dosage is 0.1%-50% and is preferably 0.5-10%. The shortcomings and defects of poor absorbability in an oral taking mode and low bioavailability of the active ingredient are overcome, an administration route is changed, and accordingly the medical absorbability and bioavailability are improved. A clinic preparation is convenient and simple to prepare, and large-scale production is easy. The preparation can be clinically used for reliving osteoarthritis, rheumatoid arthritis, a variety of fever and pain symptoms and other relevant symptoms. The percutaneous absorption preparation TY-501 prepared by the preparation method is good in stability, remarkable in anti-inflammatory effect and good in safety.