The invention discloses a membrane protein solid phase enrichment-mass spectrum detection combined system and method. The system comprises a hydrophobic porous enzyme reactor and a mass spectrometer. The hydrophobic porous enzyme reactor utilizes a porous silicon oxide material subjected to hydrophobic treatment as an adsorbent to carry out membrane protein selective enrichment and fast in-situ enzymolysis. The enzymolysis product is subjected to mass spectrometry through the mass spectrometer. Through use of limitation effects of porous silicon oxide material tunnels and chemical affinity effects of a hydrophobic surface and a membrane protein, membrane protein selective enrichment and in-situ fast enzymolysis are realized and mass spectrum direct analysis is further finished. The method has simple processes, is convenient for operation, is fast and efficient and realizes high sensitivity, high accuracy and high flux mass spectrometry of the membrane protein.