The invention relates to an anti-parasitic medicine composition and application. The composition comprises an anti-parasitic active component, wherein the anti-parasitic active component comprises left-handed praziquantel, tribendimidine and/or a tribendimidine in vivo metabolite. By mixed use of the left-handed praziquantel, the tribendimidine and/or the tribendimidine in vivo metabolite, the anti-parasitic medicine composition has the synergistic interaction effect, can effectively enhance the prevention or treatment effect and reduce the medicine use amount and the cost, and enables medicine application to be simpler. Compared with a single anti-parasitic active component medicine, the composition expands the range of an anti-parasitic spectrum, and is suitable for clonorchis sinensis and/or opisthorchis viverrini infection of human and animals. Furthermore, the composition is high in medicine compliance, high in safety and high in economy.