The invention discloses a tissue cultured rapid propagation method for Rhizoma Stahlianthi Involucrati. The method comprises the following steps: selecting a rhizome of a young and tender bud of Rhizoma Stahlianthi Involucrati which grows vigorously as an explant; sterilizing the explant by successively using a potassium permanganate solution, a chlorothalonil and thiophanate-methyl mixed solution, alcohol and a mercury chloride solution; culturing on an induction medium to form an adventitious bud; after cutting off part of lamina of the adventitious bud, cutting the adventitious bud; culturing in a proliferation medium and proliferating the adventitious bud; when the height of a subculture seedling is 3-5 centimeters, cutting off the subculture seedling and inoculating into a rooting medium for culturing; when the height of a seedling on the rooting medium is 5-8 centimeters, hardening the seedling under natural illumination of a greenhouse; taking out a test-tube seedling; cleaning the medium of the root of the test-tube seedling; soaking with a chlorothalonil solution; transplanting into a matrix obtained by mixing yellow mud, perlite and peat soil; and culturing conventionally to obtain a transplanting seedling. The test-tube seedling which has excellent characters of a mother plant can be obtained within a short time, injury to the mother plant is little, and a large number of Rhizoma Stahlianthi Involucrati seedlings are provided for large-scale production of Rhizoma Stahlianthi Involucrati.