The invention relates to a cold and hot knife working station. The cold and hot knife working station comprises a high-frequency electric knife system, a cold knife system and an integrated control system, wherein the integrated control system is used for controlling the high-frequency electric knife system and the cold knife system to convey high-frequency current and a refrigerant to a tool bit of a surgical knife device at the same time or in sequence. The cold and hot knife working station is reasonable in structure, advanced in design, safe and reliable to use, low in cost, high in integration, high in flexibility and long in service life. According to the working station provided by the invention, a cold knife and a hot knife are perfectly combined, an unexpected effect is achieved through the combination and the cold knife and the hot knife are not simply combined; the cold and hot knife working station has a surgical effect when the hot knife is used for slicing, and also has a low-temperature protection effect of the cold knife; and tissues are taken out through freezing and the hot knife is cooled, so that the surgical effect is extremely improved.