The invention discloses a toxoplasma nucleic acid rapid detection kit. The kit comprises an RPA primer probe mixed solution, a PBST solution, a flow gaging chromatography test strip, RPA lyophozyme and an RPA reaction premixed liquid, and a toxoplasma nucleic acid rapid detection method is provided. The toxoplasma nucleic acid rapid detection kit has the advantages of being constructed on the basis of molecular biology, a specific primer and a probe are designed based on the toxoplasma gene B1, the recombinase polymerase amplification-flow gaging chromatography method is applied to prepare the toxoplasma nucleic acid rapid detection kit, the sensitivity of the kit is higher than that of a nest type PCR, the specificity of the kit is high, the whole process only needs 30 min, and result judgment is very convenient; after a nucleic acid sample is obtained, the whole reaction process can be carried out in the non-laboratory environment, and the toxoplasma nucleic acid rapid detection kit can be applied to clinical diagnosis and applied to multiple fields such as food safety testing, farm on-site testing and environment assessment.