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发明公开
CN106399560A 检测CYP2C9和VKORC1基因多态性的引物、探针和试剂盒 无效 - 驳回

检测CYP2C9和VKORC1基因多态性的引物、探针和试剂盒
摘要：
本发明公开了检测CYP2C9和VKORC1基因多态性的引物、探针和试剂盒，包括CYP2C9*2、CYP2C9*3和VKORC1三个位点的基因多态性检测特异性引物序列和探针序列(SEQ ID NO.1～12)。本发明运用ARMS引物区分野生型和突变型基因，具有灵敏度高、特异性强的优点，适用于多种样本类型，检测结果准确度高。另外，ARMS引物合成快速简单，合成成本低，能够有效降低生产成本，而且扩增效果更佳。该试剂盒检测速度快，整个检测过程只需要90分钟，十分方便。整个试剂盒不包含有毒有害物质，对操作人员和环境无危害，适合临床检测等广泛使用。
摘要（英）：

The invention discloses a primer, a probe and a kit for detecting gene polymorphism of CYP2C9 and VKORC1. The primer comprises a gene detection specificity primer sequence of three loci YP2C9*2, CYP2C9*3 and VKORC1 and a probe sequence (SEQ ID NO.1-12). An ARMS primer is used to differentiate field and mutant genes, so that the primer has the advantages of high sensitivity and high specificity and is suitable for various sample types and high in detection result accuracy. In addition, the ARMS primer is quick and simple to synthesize, low in synthesis cost and better in amplification effect, and production cost can be lowered effectively. The kit is high in detection speed and quite convenient, and the whole detection process only takes 90 minutes. The whole kit does not contain toxic and harmful substances, thereby being harmless to operating personnel and environment and suitable for wide application in clinic detection and the like.

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